Ecl prime manual

Clarity Max ECL Substrate is the substrate of choice for challenging blots that require maximum levels of sensitivity. Both substrates are compatible with any horseradish peroxidase (HRP) conjugate and ideal for all western blots and both digital and film-based imaging. WesternBright Quantum provides the highest sensitivity and broadest linear dynamic range. Serial dilutions of A cell lysates were blotted and probed to detect STAT-1 protein. The blots were detected with WesternBright Quantum,or two other chemiluminescent substrates (ECL and ECL Plus, GE Healthcare). Catalog number: Thermo Scientific Pierce ECL Western Blotting Substrate is a value-priced, entry-level horseradish peroxidase (HRP) substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions. Pierce ECL Western Blotting Substrate provides reliability and performance.

Pierce™ ECL Western lotting Substrate Required materials ∤ Western blot membrane ∤ X-ray film or CCD imaging system (e.g., Invitrogen ™ iBright western blot imaging systems) ∤ Rotary or rocking platform shaker Product description Thermo Scientific™ Pierce™ ECL Western Blotting Substrate is an, enhanced luminol-. The Amersham ECL Prime reagent is developed to provide an extremely high signal with improved duration by the addition of a specific catalyst for an increased light output. The enhanced oxidation of luminol is a multi-step reaction regulated by the HRP amount. To obtain optimal sensitivity and signal duration when using Amersham ECL Prime it is. •Manual •Incremental Semi-auto •Specify the region of Blocking: Amersham ECL Prime blocking agent 2% in PBS-T Primary antibody: Rabbit anti-ERK1/2

ECL. SuperSignal Pico. ECL Prime. SuperSignal Dura. ECL Select incubated with substrate that was prepared according to the manufacturers' instructions. You can also try incubating with ECL prime/plus to boost signal but I would Is there an online manual available that details how to use the TWAIN image. The results demonstrate that, Amersham ECL Prime can be used for detection of low abundant proteins, that signals are very stable over time and cover a broad.